Running agarose gels with genomic DNA to check quantity and quality in preparation for ezRAD library construction. 5 µL of DNA was loaded into each lane. Gels are 1% agarose run for 45-60 minutes at 100V, with 3 µL of HyperLadder I.

Samples from 2014-10-24…

Gel 1 Gel 2 Gel 3

Samples from 2015-05-06…

Gel 4

Gel 5

Samples from 2015-01-14 and 2014-11-04…

Gel 6

Gel 7

Gel 8

Redo 10-24-2014 samples on another gel to see if visualization is better…

Gel 9

Samples from 08-2015 and 12-2014…

Gel 10

Gel 11

Samples selected:

Date Bleached C (n=30) Not bleached C (n=14) Not bleached D (n=16)
  1, 3, 11, 19, 5, 7, 25, 31, 39, 43, 51, 53, 57, 65, 69, 71, 77, 79, 83, 91, 109, 111, 119, 121, 123, 125, 127, 129, 131, 137 40, 44, 54, 66, 72, 80, 84, 92, 110, 122, 124, 128, 130, 132 2, 4, 12, 20, 6, 8, 26, 32, 52, 58, 70, 78, 112, 120, 126, 138
10/24/14 1,3,11,19,25,39,43,53,91,125,131 40,44,66,72,124…110,128 2,12,8,32,52,58,70,120,126,138
5/6/15 51,57,65,79,127,137 84 26
1/14/15   132  
       
11/4/14 31,71,77,83,119   4
       
11/24/14      
       
8/11/15      
       
12/17/14   54,80,92,122,130 6,20,78,112