ezRAD_DNA_check
Running agarose gels with genomic DNA to check quantity and quality in preparation for ezRAD library construction. 5 µL of DNA was loaded into each lane. Gels are 1% agarose run for 45-60 minutes at 100V, with 3 µL of HyperLadder I.
Samples from 2014-10-24…
Samples from 2015-05-06…
Samples from 2015-01-14 and 2014-11-04…
Redo 10-24-2014 samples on another gel to see if visualization is better…
Samples from 08-2015 and 12-2014…
Samples selected:
Date | Bleached C (n=30) | Not bleached C (n=14) | Not bleached D (n=16) |
---|---|---|---|
1, 3, 11, 19, 5, 7, 25, 31, 39, 43, 51, 53, 57, 65, 69, 71, 77, 79, 83, 91, 109, 111, 119, 121, 123, 125, 127, 129, 131, 137 | 40, 44, 54, 66, 72, 80, 84, 92, 110, 122, 124, 128, 130, 132 | 2, 4, 12, 20, 6, 8, 26, 32, 52, 58, 70, 78, 112, 120, 126, 138 | |
10/24/14 | 1,3,11,19,25,39,43,53,91,125,131 | 40,44,66,72,124…110,128 | 2,12,8,32,52,58,70,120,126,138 |
5/6/15 | 51,57,65,79,127,137 | 84 | 26 |
1/14/15 | 132 | ||
11/4/14 | 31,71,77,83,119 | 4 | |
11/24/14 | |||
8/11/15 | |||
12/17/14 | 54,80,92,122,130 | 6,20,78,112 |